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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Fertil Steril. 2012 Oct 25;99(2):526–532.e2. doi: 10.1016/j.fertnstert.2012.09.045

FIGURE 1.

FIGURE 1

Summary of the Rhesus macaques who underwent bone marrow transplantation with autologous green fluorescent protein (GFP)-labeled CD34+ cells after total body irradiation preconditioning resulting in long-term stable mixed bone marrow chimerism of granulocyte, lymphocyte, red blood cell, and platelet lineages (7, 9). (A) Hematopoietic stem cells were mobilized into the peripheral circulation and mononuclear cells were collected by leukapheresis. (B) Peripheral CD34+ cells were isolated from the leukapheresis product. (C) The CD34+ cells were transduced with an integrating lentiviral vector expressing GFP. (D) The macaque underwent pretransplant conditioning total body irradiation (2 × 500 cGy). (E) The CD34+ cells with GFP marking were then autotransplanted back into the macaque. (F) Fluorescent microscopy was performed on endometrial and bone marrow stromal cells cultures to detect the presence of GFP+ cells (indicating donor engraftment).