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. Author manuscript; available in PMC: 2013 Nov 13.
Published in final edited form as: Biochemistry. 2012 Oct 30;51(45):9234–9244. doi: 10.1021/bi301043k

Figure 2.

Figure 2

Analysis of full-length extension by Dpo1 and Dpo4 in the presence of fixed-conformation MC-dATPs. Both Dpo1 and Dpo4 (5 nM) were incubated with a primer/template DNA substrate (100 nM) possessing a single templating thymidine at the initial insertion point. The template sequence was designed so that only a single dATP will be inserted during accurate DNA synthesis. The reaction was initiated by the addition of a mixture of MgCl2 (1 mM) and dNTPs containing dCTP, dTTP, dGTP and either dATP, N-MC-dATP or S-MC-dATP (20 μM each). Following quenching, the products were separated by 20% polyacrylamide (w/v)/7 M urea and imaged. Time points are shown underneath each lane.