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. Author manuscript; available in PMC: 2013 Nov 13.
Published in final edited form as: Biochemistry. 2012 Oct 30;51(45):9234–9244. doi: 10.1021/bi301043k

Table 1.

Steady-state kinetic parameters for Dpo1- and Dpo4-catalyzed insertion of dATP and N-MC-dATP

kcat (min−1) KM,dNTP (μM) kcat/KM,dNTP (min−1 μM−1)
Dpo1
dATP 1.4 ± 0.1 1.4 ± 0.6 1.00
N-MC-dATP 0.86 ± 0.24 1.2 ± 0.5 0.72
S-MC-dATP 0.53 ± 0.03 1.3 ± 0.3 0.41
Dpo4
dATP 18.0 ± 1.3 10.0 ± 3.3 1.80
N-MC-dATP 4.8 ± 0.4 8.7 ± 3.0 0.55
S-MC-dATP n.d. n.d. n.d.

n.d. = not determined.