Table 2.
Crystal data and refinement parameters
| Parameter | Dpo4 North | Dpo4 South |
|---|---|---|
| X-ray source | APS | APS |
| Beamline | ID24-E | ID24-E |
| Detector | ADSC Q315 | ADSC Q315 |
| Wavelength (Å) | 0.979 | 0.979 |
| Temperature (K) | 110 | 110 |
| No. of Crystals | 1 | 1 |
| Space group | P21212 | P21 |
| Unit Cell (a, b, c; Å) | 93.6,103.4,52.5 | 52.7,101.1,100.0 |
| Resolution range (Å) | 46.8–2.90 | 47.5–2.89 |
| Highest resolution shella | (3.00–2.90) | (2.96–2.89) |
| No. of measurements | 267168 | 262183 |
| No. of unique reflections | 11041 (1130) | 21258 (1829) |
| Redundancy | 3.6 (3.5) | 3.2 (3.0) |
| Completeness (%) | 97.8 (84.2) | 96.0 (63.0) |
| R-mergeb (%) | 8.8 (70.4) | 14.0 (78.8) |
| Signal to noise (I/sI) | 9.3 (1.9) | 12.7 (1.8) |
| Model Composition | ||
| No. of amino acid residues | 341 | 682 |
| No. of water molecules | 46 | 65 |
| No. of Ca2+ ions | 4 | 9 |
| No. of template nucleotides | 16 | 29 |
| No. of primer nucleotides | 14 | 26 |
| No. of MC-dATP | 1 | 2 |
| Rfc (%) | 20.2 | 20.6 |
| R-freed (%) | 27.6 | 26.5 |
| Temperature factors | ||
| From Wilson plot (Å2) | 75.1 | 60.8 |
| r.m.s. standard deviation from ideal values | ||
| Bond lengths (Å) | 0.008 | 0.010 |
| Bond angles (°) | 1.3 | 1.4 |
| Dihedral angles (°) | 20.6 | 20.8 |
a
Values in parentheses correspond to the highest resolution shells.
b
R-merge = ΣhklΣj=1,N|〈Ihkl〉−Ihkl,j|/ΣhklΣj=1,N|Ihkl,j|, where the outer sum (hkl) is taken over unique the reflections.
c
Rf= Σhkl∥Fo,hkl|−k|Fc,hkl∥/Σhkl|Fo,hkl|, where |Fo,hkl| and |Fc,hkl| are the observed and calculated structure factors respectively.
d
Rfree idem, for the set of reflections (10% of the total) omitted from the refinement process.