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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Biochem Biophys Res Commun. 2013 Jan 3;431(1):41–46. doi: 10.1016/j.bbrc.2012.12.102

Figure 2. CD9+ GC-B cells are more advanced cells than CD9- GC-B cells in the course of GC-B cell differentiation to PC.

Figure 2

(A) CD9+ and CD9− GC-B cells were isolated with anti-CD9 antibody (10E5 clone) using a MACS column. Purity of the populations was determined by staining with FITC-conjugated anti-mouse IgG antibody. (B) Expression of transcription factors for PC differentiation was examined in isolated CD9+ and CD9− GC-B cells by a quantitative real-time PCR. The relative fold changes of each transcript in CD9+ GC-B cells are shown in comparison to the levels of the transcript in CD9− GC-B cells, which is assigned the value 1. One representative data from four reproducible experiments is shown. (C–E) CD9+ and CD9− GC-B cells were cultured for 4 days in the presence of FDC/HK cells with IL-2, IL-10, and CD40L. At the end of the culture, cell surface phenotype was determined by flow cytometry analysis after staining the cells with anti-CD38, anti-CD27, and anti-CD20. The percentage of plasmablasts (CD20-CD38+ or CD27+CD38+) is indicated (C). The numbers of CD20-CD38+ and CD27+CD38+ plasmablasts (D) and IgG concentration (E) were determined (*, P < 0.05; **, P < 0.01; ***, P < 0.001).