Fig. 3.

Gene expression of osteoblast and adipocyte markers during in vitro differentiation of hMSC-TERT, hATSCs, hASSCs and hNSSCs. The human bone marrow stromal (mesenchymal) stem cells (hMSC) immortalized with human telomerase reverse transcriptase gene (hMSC-TERT) and stromal cells derived from adipose tissue (hATSCs), adult dermal skin (hASSCs) and neonatal foreskin (hNSSCs) cells were cultured using plastic adherence and exposed to either osteoblast or adipocyte differentiation medium over a 21 day (D) period. Gene expression was normalized to beta-ACTIN and was represented as fold-change of non-induced D0 control cells. hMSC-TERT, hATSCs, hASSCs and hNSSCs data are shown as mean ± SD of three donor biological samples from at least two independent experiments. ALP = alkaline phosphatase, PPARg2 = Peroxisome proliferator-activated receptor gamma2, aP2 = adipocyte protein 2