Fig. 2.

In vitro entrainment of human islets by simulated body temperature cycles. Human islets transduced with Bmal1-luc reporters were synchronised by dexamethasone and maintained at constant 37°C temperature (black line), or continuously entrained by simulated body temperature cycles (red line). (a) Temperature measurements throughout bioluminescence recordings in dishes of dexamethasone-synchronised (black) and temperature-entrained (red) islets. (b) Raw Bmal1-luc traces of dexamethasone-synchronised (black) and temperature-entrained (red) islets. (c) Same data as in (b), which has been detrended as described in the Methods (y-axis shows detrended bioluminescence)