Fig. 3.

Oscillation of clock genes (a–i) and functional genes (j–l) in human islets. qPCR was performed in whole islets for core clock genes (a) BMAL1, (b) CRY1, (c) CRY2, (d) REV-ERBα, (e) CLOCK, (f) clock output gene DBP, (g) PER1, (h) PER2 and (i) PER3 and for islet functional genes (j) HNF1A, (k) PDX1 and (l) INSULIN transcripts, normalised to GAPDH and 9S house-keeping genes. Each time point represents 1,000 islets. Profiles are representative of three experiments (mean ± SEM), each using islets from one donor