Figure 5.

Ephrin B3 suppression in combination with IR causes senescence and concomitant alteration of Akt, MAPKs ERK, and p38 phosphorylation, and p27kip1 expression. U-1810 cells were treated as indicated in Figure 4. At 48 h post IR, the cells were stained for β-galactosidase activity as a marker of senescence. (a) Examples of senescent cells are given in the left panel. The percentage of cells out of 100 cells counted in three different fields is presented in the right panel. Data shown are the mean ±S.D. of three separate siRNA transfections. (b) Western blot analysis of Akt, p42/p44 ERK, and p38MAPK (phospho and total) (left) and p21^WAF1/Cip1, p16^INK4A and p27^Kip1, cyclin B1 and cdc2 (Tyr15) (right) at 0 or 48h post IR. Ku80 was used as a loading control. Western blot presented is one representative experiment of three performed experiments. (c) The expression of EphA4 and Ephrin A1 in U-1810 cells treated as in (b) was examined. Ku80 was used as a loading control