Figure 5.

Man A inhibits human breast tumor growth in nude mice. (a) Relative sizes of tumors (left panel) and relative progression of tumor volumes (right panel) in xenografts derived from MDA-MB-231 cells in animals treated with vehicle (Veh) and Man A (5 mg/kg body weight). (b) Relative weights of tumors isolated from mice treated with vehicle and Man A (*P<0.001). Inset, represents relative body weights of treated mice. (c) Hematoxylin and eosin (H&E)-stained sections of MDA-MB-231 xenografts treated with vehicle or Man A. Vacuoles are indicated by arrow heads. (d) Increased expression of PTEN, p27 and reduced activation of Akt (pAkt) in tumors treated with Man A. (e) Increased expression of Bip, CHOP, LC3, p62 and accumulation of ubiquitinated proteins in xenograft tumors treated with vehicle or Man A