Figure 5.

Effects of resveratrol on MTOR-dependent induction of HIF-1-responsive transcription in RPE cells. (a), (c): immunoblot analysis. RPE cells were plated in 10% serum-MEM, and the next day, the medium was changed for serum-free MEM for one day before treatment. In serum-free MEM cells were treated with indicated concentrations of resveratrol followed by either insulin (a) or serum (c). Then the cells were lysed and immunoblot for pS6 and S6 was performed. (b), (d): HIF-1-responsive luciferase induction. Cells were transfected with HRE -Luc (HIF-responsive-luciferase construct) as described previously. The next day, cells were treated with were treated with indicated concentrations of resveratrol followed by either insulin (b) or serum (d). After 16 h, cells were lysed and luciferase activity was measured