Figure 2.

The loss of Trap1 in Drosophila results in a reduction of mitochondrial function and brain dopamine levels. (a) Decreased respiration in Trap1⁴ mutant flies. Activity was measured by high-resolution respirometry in 20-day-old flies. Data are shown as the means±S.D. (n≥4 in each genotype). Significant values relative to the control are indicated by asterisks (two-tailed unpaired t test). (b) Decreased complex I protein levels in Trap1⁴ mutant flies. Whole-fly (20 days old) protein lysates were subjected to western blot analysis with the indicated antibodies. (c) Trap1⁴ mutants have lower levels of ATP compared with the controls. The ATP levels were measured using a bioluminescent assay. Data are shown as the means±S.D. (n=4 for each genotype). The statistical significance is indicated by asterisks (two-tailed unpaired t-test). (d) Analysis of TH levels in Trap1 mutant flies. Fly-head protein lysates from 20-day-old flies were used for western blotting analysis with the indicated antibodies. (e) Trap1 mutant flies have decreased dopamine and increased serotonin levels. Neurotransmitter levels were assessed by HPLC with electrochemical detection. Data are shown as the means±S.D. (n≥5 for each genotype). The statistical significance is indicated by asterisks (two-tailed unpaired t-test)