Figure 5.

Drosophila Trap1 reverses mitochondrial dysfunction in Pink1 mutants. (a) Trap1 expression restores the mitochondrial complex I protein levels in the Pink1^B9 mutant flies. Whole-fly protein lysates were used for western blotting analysis with the indicated antibodies. (b) Trap1 expression partially rescues the mitochondrial respiratory defects in the Pink1^B9 mutants. Measurements of state three respiration of complex I and complex II, and complex II uncoupled respiration by high-resolution respirometry. The data are shown as the means±S.D. (n=6 for each genotype). The statistical significance is indicated by the asterisks (one-way ANOVA with Dunnett's multiple comparison test). (c) Expression of Trap1 increased the ATP levels in the Pink1^B9 mutants. ATP levels were measured using a bioluminescent assay. Data are shown as the means±S.D. (n=4 for each genotype). The statistical significance is indicated by the asterisks (one-way ANOVA with Dunnett's multiple comparison test relative to the control)