Figure 6.

Fluorescence lifetime imaging of GSAO-AF750 bound to dying/dead cells. (a) Single exponential fluorescence lifetime analysis of GSAO-AF750 labelling of dying/dead cells in culture. Focal cell death in confluent monolayers of 4T1-luc2 mouse breast cancer cells was induced by applying dry ice to the underside of the culture well and the cells subsequently incubated with GSAO-AF750 (top left well), GSAO-AF750 with DMP (top right well), GSCA-AF750 (bottom left well) or GSCA-AF750 with DMP (bottom right well). (b) Single exponential fluorescence lifetime analysis of GSAO-AF750 labelling of dying/dead cells in brain cryolesions of two different mice