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. 1972 Jun;9(6):956–968. doi: 10.1128/jvi.9.6.956-968.1972

Genetic Analysis of Simian Virus 40 III. Characterization of a Temperature-Sensitive Mutant Blocked at an Early Stage of Productive Infection in Monkey Cells 1

James A Robb a, Robert G Martin b
PMCID: PMC356401  PMID: 4338641

Abstract

A temperature-sensitive mutant of simian virus 40 (SV40), ts*101, has been characterized during productive infection in monkey kidney cells. The mutant virion can adsorb to and penetrate the cell normally at the restrictive temperature, but cannot induce the synthesis of cellular deoxyribonucleic acid (DNA) nor initiate the synthesis of SV40-specific tumor, virion, or U antigens or viral DNA. First-cycle infection with purified ts*101 DNA is normal at the restrictive temperature, but the resulting progeny virions are still temperature-sensitive. The mutant neither complements nor inhibits other temperature-sensitive SV40 mutants or wild-type virions. The affected protein in the ts*101 mutant may be a regulatory structural protein, possibly a core protein, that is interacting with the viral DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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