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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: J Allergy Clin Immunol. 2013 Feb;131(2):491–500.e5. doi: 10.1016/j.jaci.2012.11.037

Figure 3. IgE cross-linking impairs monocyte phagocytic function.

Figure 3

A,B. Photomicrographs (A, grey=DIC, blue=DAPI, green=BODIPY-FL) and particle count per cell (B) of BODIPY-labeled bacteria phagocytosed by monocytes after 96 hr culture in indicated conditions (N=5). C. Fluorescence of internalized bacteria determined by flow cytometry for indicated conditions (N≥9). D. Effect of IL-10 neutralization on phagocytosis in indicated conditions (N=3). E. Internalization (CFU/Cell, left) of live bacteria after 48 hr culture; percent of internalized bacteria killed (right) after additional 16 hr incubation (N=4). * p<.05, ** p<.01, *** p<.001, NS p>.05 for αIgE vs. Media and IgG (C) or indicated comparisons (B,D,E).