Table 2.
Composition of experimental diets (g/kg).
| Control | Ergot 5-low | Ergot 5-high | Ergot 15-low | Ergot 15-high | |
|---|---|---|---|---|---|
| Components | |||||
| Barley | 215.2 | 209.2 | 203.2 | 197 | 178.8 |
| Wheat | 200 | 200 | 200 | 200 | 200 |
| Maize | 150 | 150 | 150 | 150 | 150 |
| Soybean meal | 200 | 200 | 200 | 200 | 200 |
| Soybean concentrate 1 | 40 | 40 | 40 | 40 | 40 |
| Hydrothermally treated maize | 100 | 100 | 100 | 100 | 100 |
| Soybean oil | 40 | 40 | 40 | 40 | 40 |
| Vitamin and mineral premix 2 | 40 | 40 | 40 | 40 | 40 |
| DL-methionine | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 |
| L-lysine mono-hydro-chloride | 4.5 | 4.5 | 4.5 | 4.5 | 4.5 |
| L-threonine | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 |
| L-tryptophan | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
| Formic acid 3 | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 |
| Phytase 4 | 0.3 | 0.3 | 0.3 | 0.3 | 0.3 |
| Ergot Batch 5 5 | 6.0 | 12.0 | |||
| Ergot Batch 15 6 | 18.2 | 36.4 | |||
| Calculated composition 7 | |||||
| Total alkaloids 8 [mg/kg] | 8.4 | 16.8 | 8.4 | 16.8 | |
| Crude protein 9 | 184 | 184 | 185 | 185 | 187 |
| Metabolizable energy (ME)[MJ/kg] 9 | 14.0 | 14.0 | 14.0 | 14.0 | 14.0 |
| Lysine 9 | 12.3 | 12.3 | 12.4 | 12.4 | 12.6 |
| Methionine and cystine 9 | 7.4 | 7.4 | 7.4 | 7.5 | 7.5 |
| Threonine 9 | 8.1 | 8.1 | 8.1 | 8.2 | 8.2 |
| Tryptophan 9 | 2.6 | 2.6 | 2.6 | 2.7 | 2.7 |
| Calcium 9 | 9.3 | 9.3 | 9.4 | 9.5 | 9.6 |
| Total phosphorus 9 | 5.9 | 5.9 | 5.9 | 5.9 | 6.0 |
| Sodium 9 | 2.2 | 2.3 | 2.3 | 2.3 | 2.3 |
| Analyzed composition 7 | |||||
| Crude ash | 64.0 | 60.3 | 61.6 | 58.3 | 62.9 |
| Crude protein | 212 | 197 | 199 | 191 | 200 |
| Crude fat | 48.6 | 40.7 | 51.1 | 54.8 | 41.6 |
| Crude fiber | 34.9 | 33.1 | 34.4 | 37.0 | 36.6 |
| ME 2 [MJ/kg] | 13.6 | 13.4 | 13.8 | 13.9 | 13.6 |
| Total alkaloids 8 [mg/kg] | <d.l. | 10.6 | 22.1 | 9.1 | 13.5 |
| Key alkaloids 10 [mg/kg] | <d.l. | 5.3 | 11.1 | 5.1 | 7.5 |
| Ergocristine [μg/kg] | <d.l. | 1848.0 | 4278.6 | 1592.6 | 2100.6 |
| Ergocornine [μg/kg] | <d.l. | 525.0 | 1098.5 | 348.0 | 584.2 |
| Ergocryptine [μg/kg] | <d.l. | 281.1 | 626.7 | 445.2 | 663.2 |
| Ergotamine [μg/kg] | <d.l. | 900.2 | 1900.1 | 1913.9 | 3025.8 |
| Ergometrine [μg/kg] | <d.l. | 1714.2 | 3185.3 | 818.1 | 1135.5 |
| Ergosine [μg/kg] | <d.l. | 1910.6 | 4240.0 | 889.5 | 1566.1 |
| ∑inine-isomeres [μg/kg] 11 | <d.l. | 3376.3 | 6840.9 | 3057.6 | 4404.7 |
1 Soycomil®, 650 g crude protein/kg, Denkavit Ingredients, Warendorf, Germany; 2 Provided per 1 kg premix: 240 g Ca; 60 g P; 55 g Na; 10 g Mg; 400,000 I.E. vitamin A; 40,000 I.E. vitamin D3; 1200 mg vitamin E; 37.5 mg vitamin B1; 100 mg vitamin B2; 100 mg vitamin B6; 750 mg vitamin B12; 52.5 mg vitamin K3; 500 mg nicotinic acid; 337.5 mg Ca-panthotenate; 5000 mg cholin chloride; 4000 mg Fe; 1000 mg Cu; 2000 mg Mn; 4000 mg Zn; 50 mg J; 15 mg Se; 20 mg Co; 3ACIDOMIX® Formic 65 G, Röthel GmbH Schwänheit 10, D-34281 Gudensberg, Deutschland; 4 ZY PHYTASE 5000 (LOHMANN ANIMAL HEALTH GmbH & Co. KG Heinz-Lohmann-Straße 4 27472 Cuxhaven Deutschland) declared phytase activity (EC 3.1.3.26): 5000 FYT/g; 5 Ergot batch contained 100% ergot; 6 Ergot batch contained 45.2% ergot and 54.8% rye; 7 Based on a dry matter content of 880 g/kg; 8 Sum of ergometrine, ergotamine, ergocornine, ergocristine, ergocryptine, ergosine and of their –inine isomers; 9 Based on table values (DLG-Futterwerttabelle, 1991); 10 Sum of ergometrine, ergotamine, ergocornine, ergocristine, ergocryptine; 11 Sum of the -inine isomers ergometrinine, ergocorninine, ergotaminine, α-ergocryptinine, ergosinine and ergocristinine. d.l.: detection limit.