. 2013 Jan 15;5(1):139–161. doi: 10.3390/toxins5010139

Table 5.

Results of the ¹³C-methacetin (MC) and ¹³C-ketoisocaproate (KICA) breath test of weaned piglets fed a control diet without ergot (Control) or diets adjusted to a total ergot alkaloid content of 16.8 mg/kg diet (Ergot 5-high; Ergot 15-high) (week 3 of experiment, n = 4).

Diet	Sex	Substrate	t_max (min)	DOB_max (‰)	t_0.5(min)	cPDR₃₀ (%)	cPDR₆₀ (%)	cPDR₁₂₀ (%)	LW at breath test (kg)	Mean LWG at breath test (g/d)
Control	Male	MC	24.3 ^abc	9.3	45.1 ^a	8.2 ^bcd	17.5 ^bc	25.0	15.8	581
Control	Male	KICA	54.4 ^c	10.5	86.2 ^ab	4.8 ^abcd	14.2 ^abc	30.5	15.0	391
Control	Female	MC	15.4 ^ab	10.5	84.0 ^ab	9.3 ^cd	18.4 ^c	30.6	14.1	485
Control	Female	KICA	38.9 ^bc	7.6	58.1 ^a	4.1 ^abc	11.0 ^abc	18.0	12.9	309
Ergot 5-high	Male	MC	26.9 ^abc	7.1	57.9 ^a	5.6 ^abcd	11.9 ^abc	18.6	12.3	344
Ergot 5-high	Male	KICA	30.3 ^abc	7.2	124.6 ^b	3.5 ^ab	9.9 ^ab	19.2	12.0	277
Ergot 5-high	Female	MC	23.6 ^abc	11.2	50.3 ^ab	8.7 ^cd	14.8 ^abc	21.6	10.2	180
Ergot 5-high	Female	KICA	42.5 ^c	6.3	54.4 ^ab	2.5 ^a	8.2 ^a	14.5	13.9	374
Ergot 15-high	Male	MC	11.3 ^a	9.3	39.5 ^ab	10.8 ^d	19.0 ^c	26.0	14.0	440
Ergot 15-high	Male	KICA	35.3 ^abc	6.4	49.5 ^ab	3.8 ^ab	10.1 ^ab	15.2	11.1	298
Ergot 15-high	Female	MC	21.9 ^abc	10.3	63.2 ^ab	8.6 ^cd	16.6 ^abc	27.7	13.3	345
Ergot 15-high	Female	KICA	34.8 ^abc	11.9	48.9 ^ab	6.5 ^abcd	16.3 ^abc	23.4	12.4	330
ANOVA (p-values)
Diet	-	-	0.523	0.357	0.255	0.211	0.130	0.219	0.056	0.069
Sex	-	-	0.870	0.206	0.550	0.685	0.815	0.954	0.453	0.286
Substrate	-	-	0.002	0.206	0.271	0.001	0.024	0.171	0.594	0.176
Diet × sex	-	-	0.407	0.289	0.159	0.937	0.829	0.589	0.491	0.874
Diet × substrate	-	-	0.538	0.717	0.375	0.989	0.981	0.826	0.095	0.133
Sex × substrate	-	-	0.949	0.461	0.043	0.889	0.991	0.355	0.208	0.170
Diet × sex × substrate	-	-	0.522	0.091	0.709	0.235	0.277	0.352	0.549	0.592
PSEM	-	-	5.7	1.1	12.8	1.3	2.1	3.6	0.8	50

Abbreviations: LW = live weight, LWG = LW gain, t_max (min) = time at the maximum ¹³C-exhalation, DOB_max (‰) = maximum delta over baseline value, t_0.5(min) = time when the half of the recovered ¹³C is exhaled, cPDR_{30, 60, 120} (%) = cumulative percent of the applied ¹³C-dose recovery after 30, 60 or 120, MC = ¹³C-methacetin, KICA = ¹³C-ketoisocaproate; PSEM = pooled standard error of means.