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. 2013 Jan 11;5(1):127–149. doi: 10.3390/v5010127

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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NB-2 inhibits 6-HB formation in a dose-dependent manner, as assessed by N-PAGE. NB-2 at different concentration was mixed with N36 at 37 °C for 30 min before addition of C34. After incubation for another 30 min, the mixture was analyzed by N-PAGE. As shown in this figure, N36 showed no band (lane 1), C34 showed a single band in the lower portion of the gel (lane 2), and the mixture of N36 and C34 showed a band in the upper portion of the gel corresponding to the band for 6-HB (lane 3). In the presence of increasing concentration of NB-2, the bands of the 6-HB formed between N36 and C34 (upper bands in lanes 4–9) become weaker and weaker than that without NB-2 (upper band in lane 3).