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. 2013 Jan 21;5(1):295–320. doi: 10.3390/v5010295

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Cytokine expression by Balb/c and C57Bl/6 mice following infection with PVM 15. Five to six week-old Balb/c and C57Bl/6 mice were inoculated with medium, 30 pfu, 300 pfu, or 3000 pfu of PVM 15 and lungs were collected from four mice per group on days 3 (A and C) and 5 p.i. (B and D). Expression levels of IFN-α (A and B) and IFN-γ (C and D) transcripts were calculated using the Bio-Rad analysis software (Bio-Rad CFX Manager Version 2.0), normalized against the expression of both β-actin and GAPDH housekeeping genes, and expressed as the normalized fold-change over mock-infected control animals euthanized on the same day p.i.. Each data point represents a single animal and the line represents the group median. *, p <0.05.