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. 2013 Jan 21;5(1):295–320. doi: 10.3390/v5010295

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Percentage of IFN-γ secreting NK cells in lungs of Balb/c and C57Bl/6 mice in response to PVM infection. Five to six week old-mice were inoculated with medium (control), or 30 pfu (Balb/c), or 300 pfu (C57Bl/6) of PVM 15 and sacrificed on day 6 p.i.. Cells collected from pooled lungs of five animals were stained with anti-CD8a-FITC, anti-CD3-PE, anti-CD4-FITC, anti-CD335(NKp46)PerCP-Cy^TM 5.5 for surface markers, followed by permeabilization and fixation with BD Cytofix/Cytoperm^TM Plus and staining with anti-IFN-γ-APC. Flow cytometry was performed using a FACS Calibur (BD Biosciences), and data analysis was performed using Kaluza software. Median values with interquartile range are shown. **, p <0.01; ***, p <0.001.