. 2013 Jan 21;5(1):295–320. doi: 10.3390/v5010295

Table 1.

Cytokine and Chemokine Primers and their Optimal Annealing Temperatures.

Target gene	Direction	Sequence	Source	Annealing Temp (°C)
β-actin	Forward	ACTGGGACGACATGGAG	[44]	57.5
β-actin	Reverse	GTAGATGGGCACAGTGTGGG	[44]	57.5
GAPDH	Forward	AACTTTGGCATTGTGGAAGG	[45]	57.5
GAPDH	Reverse	ACACATTGGGGGTAGGAACA	[45]	57.5
CCL3/MIP-1α	Forward	CTTCTCTGTACCATGACACTC	[44]	57.5
CCL3/MIP-1α	Reverse	AGGTCTCTTTGGAGTCAGCG	[44]	57.5
CXCL8/MIP-2	Forward	TGCGCCCAGACAGAAGTCATAGC	designed in house	63.9
CXCL8/MIP-2	Reverse	GCTCTAGAGTCAGTTAGCCTTGCCTTTG	designed in house	63.9
CCL2/MCP-1	Forward	CTTCTGGGCCTGCTGTTCA	[46]	57.5
CCL2/MCP-1	Reverse	CCAGCCTACTCATTGGGATCA	[46]	57.5
IFN-γ	Forward	TCAAGTGGCATAGATGTGGAAGAA	[46]	57.5
IFN-γ	Reverse	TGGCTCTGCAGGATTTTCATG	[46]	57.5
IL-4	Forward	GGAGATGGATGTGCCAAACG	designed in house	63.9
IL-4	Reverse	ACCTTGGAAGCCCTACAGAC	designed in house	63.9
IFN-α	Forward	CCTGTGTGATGCAACAGGTC	[47]	59.3
IFN-α	Reverse	TCACTCCTCCTTGCTCAATC	[47]	59.3
IFN-β	Forward	ATCATGAACAACAGGTGGATCCTCC	[47]	63.9
IFN-β	Reverse	TTCAAGTGGAGAGCAGTTGAG	[47]	63.9
TNF-α	Forward	GAACTGGCAGAAGAGGCACT	[47]	68.9
TNF-α	Reverse	AGGGTCTGGGCCATAGAACT	[47]	68.9
CXCL10/IP-10	Forward	GAGATCATTGCCACGATGAA	designed in house	63.9
CXCL10/IP-10	Reverse	CACTGGGTAAAGGGGAGTGA	designed in house	63.9
CCL5/RANTES	Forward	CTCACTGCAGCCGCCCTCTG	designed in house	57.5
CCL5/RANTES	Reverse	CCTTGACGTGGGCACGAGGC	designed in house	57.5
CCL11/Eotaxin	Forward	AGAGGCTGAGATCCAAGCAG	designed in house	63.9
CCL11/Eotaxin	Reverse	CAGATCTCTTTGCCCAACCT	designed in house	63.9