Figure 4.

Propagation of cytosolic prions derived from the S. cerevisiae Sup35 prion domain NM. N2a cells ectopically express the HA-tagged prion domain NM of Sup35, which is the most well characterized yeast prion. The left image shows aggregated NM-HA (green) after induction with recombinant NM fibrils, the right image shows the soluble NM-HA (green). NM was stained with anti-HA antibody. F-Actin was stained with fluorescently conjugated phalloidin (red). Nuclei were stained with Hoechst (blue). Scale bar: 5 µm.