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. 2013 Jan 22;5(1):374–405. doi: 10.3390/v5010374

Table 1.

Cell culture models susceptible to transmissible spongiform encephalopathy (TSE) agents.

Cell designation Tissue of origin or cell type Species of origin Prion strain References
1. Neuronal or brain-derived cells
N2a neuroblastoma cell line* mouse Chandler, RML, 139A, 22L, C506, Fukuoka-1, FU CJD [95,96,127,139,140,141,142,143,144]
GT1 hypothalamic cell line mouse Chandler, RML, 139A, 22L, kCJD, FU CJD , M1000 [96,97,99,120,139,145]
SN56 cholinergic septal cell line mouse Chandler, ME7, 22L [146]
HpL3-4 hippocampal PrP-deficient cell line,
upon ectopic expression of moPrP*		 mouse 22L [121,147]
CF10 brain derived PrP-deficient cell line,
upon ectopic expression of moPrP		 mouse 22L [122]
SMB prion-infected brain cell mouse Chandler, 139A, 22F, 79A [93,148,149]
CAD catecholaminergic cell line mouse RML, 22L, 22F, 79A, 139A, ME7 [125,150,151,152]
MG20 microglial cell line overexpressing PrPC tg20 mouse Chandler, ME7, Obihiro, mouse-adapted BSE [98]
PC12 pheochromocytoma cell line rat 139A, ME7 [153,154,155]
HaB brain-derived cell line hamster Sc237 [131]
SH-SY5Y neuroblastoma cell line human sCJD brain material [119]
MDB primary brain cells,
SV40 transformed		 mule deer CWD [129]
2. Primary neuronal or brain-derived cells
CGN cerebellar granule neurons overexpressing ovine PrPC tgov mouse mo 127S [111]
CAS cerebellar astrocytes overexpressing ovine PrPC tgov mouse mo 127S [111]
NSC neural stem cells mouse 22L, RML [112,113,115]
3. Non-neuronal cells
C2C12 skeletal myoblast cell line mouse 22L [108]
L fibroblasts fibroblast cell line mouse ME7, Chandler [106]
L929 fibroblast cell line mouse 22L, RML, ME7 [107]
NIH/3T3 fibroblast cell line mouse 22L [107]
MSC-80 Schwann cell line mouse Chandler [156]
MovS Schwann cell-like from dorsal root ganglia tgov mouse PG127, SSBP/1, scrapie field isolates [104,157]
moRK13 epithelial cell line expressing mouse PrPC rabbit Fukuoka-1, 22L, Chandler, M1000, mo sCJD [99,100,101,120]
voRK13 epithelial cell line expressing vole PrPC rabbit vo BSE [100]
ovRK13/ RoV9 epithelial cell line expressing ovine PrPC rabbit PG127, LA404, SSBP/1, scrapie field isolates [102,103,104]
elkRK13 epithelial cell line expressing elk PrPC rabbit CWD [105,109]
4. Primary non-neuronal cells
BM-derived MSC bone marrow derived mesenchymal stem cell mouse Fukuoka-1 [110]
BM-derived MSC-like bone marrow derived mesenchymal stem cell like mouse Fukuoka-1 [114]

* some cells overexpress moPrPC-A or 3F4 antibody-epitope tagged moPrPC