Table 2.
Routes of prion dissemination in cell culture.
| Prion-infected donor cell line | Prion strain | Intercellular prion spreading | PrPSc secreted | References |
|---|---|---|---|---|
| N2a | 22L | Yes, via conditioned medium | Yes, associated with exosomes | [36] |
| N2a | RML | No or inefficient | Not determined | [97,196] |
| SMB | Chandler | Yes, via direct cell contact | Not determined | [149] |
| HpL3-4* | 22L | Yes, via conditioned medium | Not determined | [121] |
| NIH/3T3 | 22L | Yes, via conditioned medium | Yes, associated with exosomes | [198] |
| CAD | 139A | Yes, via TNTs | Not determined | [200] |
| GT1 | RML | Yes, via conditioned medium | Not determined | [97] |
| GT1 | FU CJD | Yes, via conditioned medium | Not determined | [201] |
| GT1 | M1000 | Yes | Yes, associated with exosomes | [99] |
| ovRK13/ RoV9 | PG127 | Yes (inefficiently) | Yes, associated with exosomes | [37,197] |
| moRK13 | M1000 | Yes | Yes, associated with exosomes | [99] |
| Mov | PG127 | Yes, via close proximity of cells | Yes, associated with exosomes | [37,111,197] |
| SN56 | Chandler | Yes, via conditioned medium | Yes | [202] |
* cells ectopically express 3F4 antibody-epitope tagged moPrPC