Table 1.
Analysis of cell cycle distribution. Cells were treated with FE, CDDP, TAM or TAXOL alone or in combination with FE for 48 h. Cells were then measured by flow cytometry as described in the Methods section. The cell distribution was analyzed by flow cytometry analysis software.
| Treatment | MDA-MB-231 | MCF-7 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| G1 | S | G2/M | Sub-G1 | G1 | S | G2/M | Sub-G1 | ||
| Control | 65.9 ± 2.8 | 21.9 ± 2.0 | 12.2 ± 1.8 | 1.9 ± 0.2 | 54.6 ± 5.2 | 31.7 ± 2.5 | 13.7 ± 0.5 | 5.0 ± 0.2 | |
| FE (200 μg/mL) | 68.7 ± 1.0 | 18.8 ± 1.6 | 12.5 ± 0.9 | 10.7 ± 0.8 | 57.3 ± 4.8 | 27.5 ± 1.5 | 15.2 ± 1.2 | 13.6 ± 1.0 | |
| CDDP (5 μM) | 13.7 ± 0.3 | 29.4 ± 3.5 | 56.9 ± 3.2 | 17.5 ± 4.3 | 37.1 ± 1.4 | 14.1 ± 1.0 | 48.8 ± 1.5 | 8.9 ± 1.5 | |
| CDDP + FE | 19.9 ± 0.7 | 21.4 ± 0.6 | 58.7 ± 0.4 | 37.8 ± 6.2 | 26.2 ± 2.5 | 29.0 ± 2.6 | 44.8 ± 2.0 | 32.4 ± 3.6 | |
| TAM (10 μM) | 66.6 ± 3.6 | 23.3 ± 2.1 | 10.1 ± 1.6 | 4.9 ± 0.6 | 63.7 ± 2.2 | 18.9 ± 1.5 | 17.4 ± 0.9 | 12.6 ± 0.9 | |
| TAM + FE | 60.8 ± 2.0 | 22.7 ± 1.5 | 16.5 ± 1.5 | 18.3 ± 2.1 | 67.8 ± 2.4 | 17.6 ± 1.6 | 14.6 ± 1.1 | 46.5 ± 4.3 | |
| TAXOL (2.5 nM) | 54.5 ± 2.0 | 20.5 ± 1.5 | 25 ± 0.8 | 6.4 ± 0.4 | 54.2 ± 2.2 | 16.9 ± 1.3 | 29.1 ± 0.8 | 8.4 ± 0.6 | |
| TAXOL + FE | 53.7 ± 1.9 | 22.1 ± 2.1 | 24.2 ± 1.6 | 25.8 ± 2.4 | 49.8 ± 2.6 | 21.8 ± 1.0 | 28.4 ± 0.7 | 20.6 ± 0.3 | |