Table 2.
Summary of results of quorum sensing inhibition bioassay of fractions eluted using five different cartridges (Bond Elut C2, C18, CN-E, PH and PPL). The results of bioassay were expressed as: (+) denotes slight (faint) effect, + denotes minimal effect, ++ denotes medium effect, +++ denotes highest effect and − denotes absence of effect. “Fing” denotes finger like projection. The bioassays were performed in triplicates. SPE: solid phase extraction.
| Bioassay results of fractions of Asparagopsis taxiformis extract | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Bond Elut SPE cartridges | Effects on plate based bioassay | Methanol (v/v) | |||||||||
| 10% | 20% | 30% | 40% | 50% | 60% | 70% | 80% | 90% | 100% | ||
| C2 | QS inhibition | (+) | − | − | (+) | − | − | (+) | − | − | + |
| Zone of clearance | − | − | − | − | ++ | +++ | − | − | − | − | |
| C18 | QS inhibition | + | − | − | − | − | + | − | − | +fing | (+)fing |
| Zone of clearance | − | − | − | − | − | − | ++ | +++ | − | − | |
| CN-E | QS inhibition | − | − | − | − | − | − | − | − | − | − |
| Zone of clearance | − | − | ++ | ++ | ++ | − | − | − | − | − | |
| PH | QS inhibition | − | − | − | − | ++ | − | − | ++ | (+)fing | +fing |
| Zone of clearance | − | − | − | − | − | ++ | ++ | − | − | − | |
| PPL | QS inhibition | +fing | − | − | − | − | − | − | (+)fing | +fing | ++fing |
| Zone of clearance | − | − | − | − | − | − | − | − | − | − | |