Table 1. Physiological parameters of rats subjected to MCAO ± HOE-642 ± Bumetanide.
| Vehicle | HOE-642 | HOE-642+Bumetanide | |
|---|---|---|---|
| Na+ (mmol/L) | 134.8±1.7 | 133.6±0.9 | 139.7±3.8 |
| K+ (mmol/L) | 5.7±0.7 | 5.5±0.7 | 6.1±2.9 |
| Cl− (mmol/L) | 103.8±2.4 | 101.6±1.6 | 105.3±4.2 |
| pH | 7.30±0.50 | 7.37±0.04 | 7.3±1.23 |
| pCO2 (mm Hg) | 47.6±7.8 | 42.1±4.5 | 44.5±4.2 |
| HCO3− (mmol/L) | 23.8±1.2 | 24.4±2.2 | 23.0±1.0 |
| BUN (mg/dL) | 41.8±3.7 | 43.4±2.5 | 44.3±1.2 |
| Glucose (mg/dL) | 192.0±46.8 | 204.0±26.4 | 164.3±28.2 |
| Hemoglobin (g/dL) | 13.8±1.5 | 14.2±0.9 | 13.0±0.0 |
| Hematocrit (%PCV) | 40.0±4.2 | 41.2±2.5 | 38.7±0.5 |
| MAP (mm Hg) | 87.6±6.00 | 84.8±2.80 | 90.1±20.06 |
BUN, blood urea nitrogen; MAP, mean arterial pressure; PCV, packed cell volume; pMCAO, permanent middle cerebral artery occlusion.
Before MCAO, rats were nephrectomized, treated with vehicle or HOE-642 (15 mg/kg) or HOE-642 plus bumetanide (15 mg/kg each). All physiological parameters shown except MAP were determined by iStat after 3 hours of permanent MCAO as described in Materials and Methods. Values are mean±s.d. for 6, 5, and 3 experiments with Vehicle, HOE-642, and HOE-642 + Bumetanide, respectively. MAP measurements were taken every 30 minutes over imaging period and a single average MAP value obtained for each animal. Variation of MAP within each experiment was 15.1%, 13.1%, and 17.2% of mean (s.d. as % of mean) for Sham, MCAO + Vehicle and MCAO + Bumetanide, respectively. No significant differences were found among any of the groups for any of the parameters by analysis of variance or two-tailed unpaired t-tests. P values for analysis of variance analyses were 0.1806, 0.1566, 0.1689, 0.2014, 0.3495, 0.5694, 0.4700, 0.3792, 0.3672, 0.5744, and 0.8885 for Na, K, Cl, pH, pCO2, HCO3, BUN, Glucose, Hemoglobin, Hematocrit, and MAP, respectively.