Figure 5.

Autonomously functioning sense and respond system. (A) Schematic depicts nanofactories assembled onto EGFRs on surface of PCI-15B cells. These cells, in turn, are cultivated on an inverted transwell insert exposing cell surfaces and nanofactories to the bacterial cells initially seeded on the bottom of the transwell apparatus. The bacteria then migrate vertically toward mammalian cells and subsequently initiate gene expression by sensing the in vitro AI-2 gradient, which is locally synthesized by the NF located on the mammalian cell surfaces. (B) After 9 h, bacteria and AI-2 levels were assayed in the upper chamber. (C) PCI-15B cells were rinsed and both QS-activated bacteria (DsRed) and NF (AF488) were imaged and assayed (ImageJ, D). Source data for this figure is available on the online supplementary information page.

Source data for Figure 5B ^{(9.6KB, xlsx)}

Source data for Figure 5D ^{(13.2KB, xlsx)}