Figure 5.

Identical duplicated genes contribute to fine control. (A) Growth rates and protein synthesis rates of the allele deletion strains compared to those of the wild-type strain. (B) Mapping of the data points from (A) onto the corresponding R^J and R^Sp plots (from Figures 1 and 3). (C) The relative amounts of the respective translation factors were determined as band intensity ratios in western blots using hexokinase as internal standard. (D) Competitive growth experiments yielded percentage survival rates for each single deletion strain remaining in the total population of cells. (E) RT–qPCR and northern blots (F) to determine transcript levels in the different single deletion strains compared to the wild-type strain. In (F), three different amounts of each sample (800, 400, and 200 ng total RNA) were applied to the gel to ease comparison. The average intensities of the respective northern blot bands relative to the wild-type levels (normalized to SCR1) are given in parentheses—these match closely the normalized values from RT–qPCR (E).