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Experimental timeline of the ovalbumin (OVA)-sensitized BALB/c mouse model. Six-week-old female BALB/c mice were fed with live or heat-killed Lactococcus lactis A17 (A17) for 4 weeks and intraperitoneally injected three times at days 7, 11, and 14 with 50 μg of OVA in 100 μL of Al(OH)3. Serum was collected weekly for immunoglobulin measurement. On day 28, mice were sacrificed and spleens were removed for spleen cell preparation.
