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Influence of CM on GST activity and expression in H9c2 cells. Cells were treated with DOX (0.1 μM) and/or pummelo (CM) at concentrations indicated in the Figure. (a) GST activity measurements were performed using total cell lysate as described in Materials and Methods. (b) Western blot analysis of GSTP expression in H9c2 treated with vehicle (CTRL), CM, DOX, or CM and DOX (CM + DOX) for 48 h. *P < 0.05 versus vehicle treated cells (CTRL); ◆ P < 0.05 versus DOX.
