Figure 6. Loss of cellular NADPH might trigger redox collapse.
Strain DBY746 was cultivated under standard (full circles) or caloric restriction (open squares) conditions as described in Figure 1. At the time points indicated, samples were taken for (A) whole cell analysis of GSH and GSSG levels or (B) NADPH/NADP+ measurements. The glutathione redox potential EGSH was calculated using the Nernst equation. Data points are the average of at least three independent experiments: bars indicate standard deviation.