Table 2.
Chi-square analysis of amino acid type distribution in sequence fragments containing the identified thiol group according to clusters
| Clusters | A–B | C | D–E | F | G |
|---|---|---|---|---|---|
| Table 2A | |||||
| A–B | 1 | ||||
| C | 2.5E-19 | 1 | |||
| D–E | 3.4E-28 | 0.0227 | 1 | ||
| F | 0.4871 | 4.7E-07 | 0.0001 | 1 | |
| G | 1.9E-18 | 0.0300 | 0.4358 | 0.0085 | 1 |
| Table 2B | |||||
| A–B | 1 | ||||
| C | 6.0E-05 | 1 | |||
| D–E | 0.5066 | 0.0113 | 1 | ||
| F | 0.3781 | 0.0003 | 0.1248 | 1 | |
| G | 0.2757 | 0.0169 | 0.4025 | 0.6238 | 1 |
Table 2A. Amino acid distribution was analyzed in the sequence fragments spanning five amino acids up- and downstream of the cysteine thiol (Figure 4), whose oxidation status was determined by OxICAT. The identified cysteine thiol was not included in the analysis. Table 2B. Chi-square analysis of the amino acid type distribution in the same sequence fragments analyzed in Table 2A removing any cysteines from our analysis. p-values obtained from the chi-square analysis of distribution of different amino acid types, positively and negatively charged, polar, non-polar, aromatic amino acids and cysteines (for Table 2A only) in clusters A through G (Figure 3). Degrees of freedom are 5 (Table 2A) and 4 (Table 2B), respectively. Significantly different distributions are shown in bold (p<0.05, a = 0.95).