Figure 6. .

Hyperoxia treatment differentially regulates VEGFR2 expression in NV and neural retina. (A) OIR mice were treated with hyperoxia (75% oxygen, HT) for 24 hours at P17. VEGFR2 mRNA in the retinas (left panel) and pooled retinal vessels (right panel) were quantified by qPCR and normalized to OIR mice kept in room air (control; n = 4 to 6 mice). *P < 0.05 compared with control. (B) OIR mice were treated with hyperoxia (75% oxygen, HT) for 6 and 12 hours at P17 and retinal flatmounts were stained with isolectin B4 (red) and anti-VEGFR2 (green). Representative confocal images of NV tufts (marked with white asterisk) in the central retina are shown (×400, n = 4 mice). Scale bar, 20 μm.