Figure 5. .

Decreased ZO-1 levels in cbs^−/− mouse retina. Retinal cryosections from (A) cbs^+/+ and (B) cbs^−/− mice were incubated with an antibody against ZO-1 (green), a marker of tight junctions, and isolectin-B4 (red) to label blood vessels. Scale bar: 40 μm; abbreviations for retinal layers are the same as for Figure 1. (C) Retinal protein was extracted and subjected to SDS-PAGE, followed by immunoblotting to detect Z0-1 (molecular weight ([M_r] = 198 kD); β-actin was used as the loading control. (D) Quantification of immunohistochemical fluorescence intensity (***P < 0.001, n = 6). (E) Quantification of densitometric scans of protein bands shown in (C) (*P < 0.05).