Table. .
Primary and Secondary Antibodies Used to Analyze Retinal Vasculature
|
Primary Antibody and Catalog Number |
Vendor |
Concentration |
Use as Marker |
Secondary Antibody |
| Griffonia simplicifolia isolectin-B4 (B-1105) | Vector Labs, Burlingame, CA | 7.5 μL/mL | Marker for vessels to detect vessel patterns | Texas Red avidin, 7 μL/mL; Vector Labs |
| CD105 (endoglin) rat antimouse (lot 75619) | BD Biosciences, San Jose, CA | 1:25 | Marker for angiogenesis | Alexa Fluor 555 (donkey antirat); Invitrogen, Eugene, OR |
| VEGF mouse monoclonal IgM (AB 38909) | Abcam Corp., Cambridge, MA | 1:250 | Marker for angiogenesis | Alexa Fluor 488 (donkey antimouse); Invitrogen, Eugene, OR |
| GFAP rabbit polyclonal (lot 00019620) | DakoCytomation, Carpenteria, CA | 1:100 | Marker for stressed Müller cells and astrocytes | Alexa Fluor 488 (goat antirabbit); Invitrogen, Eugene, OR |
| ZO-1 Rb.PAbzo-1 tight junction (AB 59720) | Abcam, Cambridge, MA | 1:250 | Marker for cellular tight junctions | Alexa Fluor 488 (donkey antirabbit); Invitrogen, Eugene, OR |
| Occludin rabbit polyclonal (AB 31721) | Abcam, Cambridge, MA | 1:200 | Marker for cellular tight junctions | Alexa Fluor 488 (donkey antirabbit); Invitrogen, Eugene, OR |
| IgM mouse monoclonal (AB 18401) | Abcam, Cambridge, MA | 1:200 | Isotype control (VEGF study) | Alexa Fluor 488 (donkey antimouse); Invitrogen, Eugene, OR |