FIG. 4.

In vitro differentiation potential of reprogrammed cell lines. (A) Embryoid bodies forming 5 days after equine iPSCs (line H, passage 12) were placed in suspension culture. (B–H) Immunostaining of differentiated cells for markers of ectoderm (B–D), mesoderm (E, F), or endoderm (G, H). Secondary antibody was conjugated to Alexa Fluor 568 (red) or Alexa Fluor 488 (green), and DAPI was used for nuclear counterstaining (blue). The second and fourth columns of the panel display the merged images for Alexa Fluor and DAPI. Scale bar=20 μm. Color images available online at www.liebertpub.com/scd