Table 1.
Primer | Sequence | Product (bp) |
---|---|---|
OCT4 (For) | ATTGAGACCCGAGTGAGAGG | 74 |
OCT4 (Rev) | CTGATCTGCTGCAGTGTGG | |
SOX2 (For)a | CACCCACAGCAAATGACAGC | 252 |
SOX2 (Rev)a | TTTCTGCAAAGCTCCTACCG | |
NANOG (For)b | TCCTCAATGACAGATTTCAGAGA | 323 |
NANOG (Rev)b | GAGCACCAGGTCTGACTGTT | |
DNMT3b (For) | CTTCTGCGTGGAGTGTCTGG | 169 |
DNMT3b (Rev) | GGTGTCGCTGGTAAAGAAGG | |
LIN28 (For) | CATGGGCTCTGTGTCAAACC | 167 |
LIN28 (Rev) | CGGTCATGGACAGGAAGC | |
REX1 (For) | GACGGGAAAGGCCTGGATAGAAG | 297 |
REX1 (Rev) | GGCGGTAAGAAGCTGTTGAGAAAGG | |
GAPDH (For) | CATCATCCCTGCTTCTACTGG | 117 |
GAPDH (Rev) | TCCACGACTGACACGTTAGG | |
Viral Common (For) | GGATCCCAGTGTGGTGGTACG | |
Viral Oct4 (Rev) | CTGTAGGGAGGGCTTCGGGCACTT | 850 |
Viral Sox2 (Rev) | TCACATGTGCGACAGGGGCAG | 1,000 |
Viral Klf4 (Rev) | TTAGGCTGTTCTTTTCCGGGGCCACGA | 1,200 |
Viral c-Myc (Rev) | TTATGCACCAGAGTTTCGAAGCTGTTC | 1,400 |