Figure 3. FK506 derived from FK506 treated DC causes activated T cell death in vitro.
(A) Annexin V stained CFSE labeled allogeneic CD4+ T cells cultured with DC or FKDC ± IL2 for 4 days. Boxes indicate gating strategy for selecting undivided or divided T cells in Figure 3B and C. (B) Histogram of annexin V staining of undivided or divided T cells cultured with allogeneic DC or FKDC ± IL2. Percent annexin V positive divided cells. (C) Histogram of bcl-xl staining of undivided or divided T cells cultured with allogeneic DC or FKDC ± IL2. Percent bcl-xl positive divided cells. (D) CD8 T cell IFNγ Elispot response to DC or FKDC presenting FLU infected apoptotic cells supplemented with IL2. Data are mean SFC + standard deviation of triplicate wells. p values were obtained using two-tailed unpaired t-test. Data are representative of three independent experiments.