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. 2012 Aug 16;22(4):668–678. doi: 10.1089/scd.2012.0226

FIG. 2.

FIG. 2.

Repressed BMP/Smad and activated NF-κB signaling pathways in BMMSCs from SLE patients. (A) Clustering analysis of genes in BMP/TGF-β signaling pathway in BMMSCs from SLE patients and healthy controls. (B) Western blotting analysis of phosphorylated Smad1/5/8 of BMMSCs from SLE patients and healthy controls. Cells were starved (the left) and incubated with BMP-2 (100 ng/mL) for 30 min (the right). (C) Immunoprecipitation (IP) analysis of BMMSCs from SLE patients and healthy controls. (D) Western blotting analysis of P IKKβ, t IKKβ, and IκB in BMMSCs from SLE patients and healthy controls. (E–H) Quantity analysis of western blotting analysis. *P<0.05 and **P<0.01 versus normal control groups. Nor, normal controls, SLE, systemic lupus erythematosus, Nor+BMP-2, normal BMMSCs stimulated with BMP-2; SLE+BMP-2, SLE-BMMSCs stimulated with BMP-2; TGF-β, transforming growth factor-β; NF-κB, nuclear factor κB; IκB, inhibitor κB; IKKβ, inhibitor κB kinase β.