TABLE 1.
Summary of findings
| Molecular and serologic characterization | Case 1 (HPA-22 [Sey]) | Case 2 (HPA-23 [Hug]) |
|---|---|---|
| Maternal serum versus healthy donor GPIIb/IIIa (HPA-1a/a, -3a/a) | ||
| Flow cytometric analysis | IgG−/IgM− | IgG−/IgM− |
| MACE (AP2 capture of GPIIb/IIIa) | − | − |
| Maternal serum versus healthy donor GPIIb/IIIa (HPA-1b/b, -2a/a, -3b/b, -5a/b, -15a/b) | ||
| Flow cytometric analysis | IgG−/IgM− | IgG−/IgM− |
| MACE (AP2 capture of GPIIb/IIIa) | − | − |
| Maternal serum versus paternal GPIIb/IIIa | ||
| Flow cytometric analysis | IgG+/IgM+ | IgG+/IgM− |
| (AP2 capture of GPIIb/IIIa) | − | − |
| MACE (AP2 capture of GPIIb/IIIa) | + | + |
| Mother/Father blood type | O/A1 | O/A2B |
| MACE reactivity after absorption of anti-A or anti-B | + | + |
| Molecular characterization | ||
| Exon/nucleotide change | 5/A584C | 12/C1942T |
| Amino acid change | K164T | R622W |
| Protein | GPIIb | GPIIIa |
| NCBI dsSNP accession number | rs142811900 | rs139166528 |
| Maternal serum vs. recombinant GPIIb/IIIa mutated to express paternal SNP | ||
| Flow cytometric analysis with transfected CHO cell lines | + | + |
| MACE with mutated GPIIb/IIIa (AP2/Tab capture) | + | + |