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. Author manuscript; available in PMC: 2013 Feb 5.
Published in final edited form as: Thromb Haemost. 2011 Nov 24;107(1):80–87. doi: 10.1160/TH11-08-0542

Figure 2. Immunoprecipitation analysis of anti-Seca.

Figure 2

Paternal and maternal platelets were surface labelled with biotin and lysed. Labelled platelet lysates were precipitated with control serum (lanes 1), anti-HPA-1a antibodies (lanes 2) and maternal serum (lanes 3). Immunoprecipitates were run on 7.5% SDS-PAGE under non-reducing conditions, transferred onto nitrocellululose membrane and visualised by streptavidin-chemiluminescence system.