Figure 3. Nucleotide sequencing analysis of amplified β3 DNA.

A) Genomic DNA derived from paternal platelets was amplified by PCR. The analysis of PCR product encompassing nucleotides 1801 – 1818 is presented. The wild-type β3 isoform Asn₅₈₀ (AAT) and the mutated β3 isoform Lys₅₈₀ (AAG) are indicated (*). B) Nucleotide sequence alignment of β3 integrin (residues 568 – 584) from human (Hu), monkey (Mo) and canine (Ca) genes. The position associated with the Sec^a phenotype is indicated. Two adjacent point mutations, S₅₇₉ and S₅₈₁, associated with functional defects of the αIIbβ3 receptor are shown.