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. Author manuscript; available in PMC: 2013 Feb 5.
Published in final edited form as: Thromb Haemost. 2011 Nov 24;107(1):80–87. doi: 10.1160/TH11-08-0542

Figure 4. Analysis of CHO cells transfected with β3 allelic constructs and αIIb expression vector.

Figure 4

A) Flow cytometry analysis of stably transfected cells expressing wild-type and mutant β3 isoforms with normal serum (Ctrl), mab Gi5 against αIIbβ3, and anti-Seca antibody. The expression of αIIbβ3 was comparable between the two cell lines (mean fluorescence intensity (MFI; mean ±SD) for Asn580 = 557 ±89 and MFI for Lys580 = 702 ±74). Bound antibody was detected with fluorescein-labelled secondary antibodies. B) Immunoprecipitation analysis of stably transfected cells expressing wild-type (lanes 1) and two different clones (clone 2 and clone 13) expressing mutant β3 isoform (lanes 2 and 3) with normal serum (Ctrl), anti-HPA-1a antibody and anti-Seca antibody as indicated.