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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: J Proteome Res. 2013 Jan 16;12(2):559–572. doi: 10.1021/pr300869h

Figure 3. Schematic depiction of the methods used in this analysis.

Figure 3

C8166 T-cells were infected with HIV-1 + VSVg pseudotyped virus for 20 hours. Cells were lysed by hypotonic swelling and Dounce homogenization. Lysates were loaded on a 5–45% sucrose gradient and subjected to ultracentrifugation for 1 hour at 207,570×g. Gradients were fractionated and digested in solution with trypsin prior to MS/MS analysis. For each infected replicate, a parallel uninfected sample was subjected to the same procedure.