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. 2013 Jan 23;33(4):1462–1485. doi: 10.1523/JNEUROSCI.3206-12.2013

Figure 6.

Figure 6.

Effect of microglial age in the proliferation, neuron differentiation, and Caspase3 activation of NPCs derived from young, middle-aged, and aged SVZ. NPCs were cocultured with purified microglia acutely isolated from mice of the indicated ages, or with a nonspecific insert. A, B, Comparison of representative confocal images of dual staining with IBA1 (green) and Map2a (red) counterstained with DAPI (blue) in young NPCs cocultured with microglia of 2 d (2d), 2 months (2M), or 24 months (24M) (A); or aged NPCs cocultured with 2-d-old (2d), 2-month-old (2M), or 24-month-old (24M) microglia (B). C, D, Differences in NPC proliferation and neuron differentiation assessed with Map2a in young (C) and aged (D) NPCs. E, Caspase3-like activity, assessed with the fluorogenic substrate DEVD-AFC in NPCs cocultured with microglia with the indirect coculture paradigm, shows increased activity as a function of microglia age. Results are expressed as percentage changes relative to controls. Three independent cultures were used for quantification. *p < 0.05 versus nonspecific insert; °p < 0.05 versus 2-d-old and 2-month-old microglia. Caspase3-like activity was increased in young and aged NPCs cocultured with aged microglia. NSI, nonspecific insert.