. 2013 Feb 1;24(3):385–397. doi: 10.1091/mbc.E12-06-0477

TABLE 3:

Primers used in this study.

Primer	Sequence (5¢–3¢)^a	Purpose and features
1	CGGGATCCCGTGCTCAGCCGTTGATAG	pPR673-HOG1
2	GGCGACGCGTCGAGCTCCGTTGGCGGAATC
3	CGGGATCCCATGTCCAGTGACGTAAGTATA	pPR673-SKO1
4	GGCGACGCGTCGTGTAGGATTTAAAGTAGTTGG
5	CAACATTTTAAACAAGTTATAGAAAGAAAATTTTTACAAAGATAAAGCATATAAGAAAATTGTGGAATTGTGAGCGGATA^b	HOG1 disruption
6	ATAGTAATACATATTTCACTTTTAAATTTATTTCTATAATTGCTAGCTTGTATTTTTGAATTTCCCAGTCACGACGTT^b
7	CTATTACATTACTTATATCTTATTGTTCTACGCGTGTGTTACTTCCCCACTTCTGTTTTCACCATTGTGGAATTGTGAGCGGATA^b	PTP2 disruption
8	GAATATTTATACTTGATTCTGTTGATAATCTTTTTCATGATTAATTAAAAATTTTATAAAAATTTCATAACTTTCCCAGTCACGACGTT^b
9	TCTGACAGGTGCTAATGACTC	PTC1-qRT PCR
10	GGCAATGTATGTGTGGACATC
11	AAGCTGCTATTTTCACTGGC	PTC2-qRT PCR
12	GCACATCCACTATCATCATC
13	ACCTTGTTGTATGTCATG	PTP2-qRT PCR
14	GTTTGTAGAAGCTGTTAG
15	GATATTAGACCATCTGCTC	PTP3-qRT PCR
16	CAGCAAATCTATCTCTCTG
17	CGAGGTAGACAGTGTTCAG	DAL5-qRT PCR
18	CCAAGCACTGACGCATAAC
19	CTAGGGCCCCTCAGCTAGTAACACTACTG	pSFS2-HOG1
20	GTACTCGAGCTCCATCTGCAGACATTTTC
21	CTAGCGGCCGCCAACGGAGCTTAATCTTC
22	TCCCCGCGGGGAACCTGATGTTAGTTG
23	CAATAATAATAGTAATAATAATAGATTCAATTCAGTTTCAGTTTCAATTTCAATTTCAATTTCAATTTGTGGAATTGTGAGCGGATA^b	PTC2 disruption
24	CAAAATCAAAATTAAAATCAAAATTCATTCCGTTACTTAAAAATTTTTATTTTTTTTTTAATTCTTTCCCAGTCACGACGTT^b

^aRestriction sites are underlined.

^bBoldface sequences in primers are segments that anneal to plasmids pGEM-HIS1, pGEM-URA3, and pRS-ARG4ΔSpeI for amplification of disruption cassettes.