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. 2013 Feb 1;24(3):409–420. doi: 10.1091/mbc.E12-07-0516

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© 2013 Yerko et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — The RBL^Ste11 domain is essential for pheromone response signaling. (A) Schematic diagram of Ste11 MAPKKK with functional domains indicated: KD, kinase domain; RBL, RBD-like; SAM, sterile alpha mating. (B) The RBL^Ste11 domain is essential for mating. Yeast cells (ste11Δ) transformed with STE11 alleles were tested for their ability to mate with tester strain by diploid selection. (C) The RBL domain is not required for Ste11 signaling in the HOG pathway. Yeast cells (ste11Δ ssk2Δ ssk22Δ) transformed with STE11 alleles were tested for their ability to signal in the HOG pathway as indicated by growth on hyperosmotic medium. Halo assay (D) and β-galactosidase assay (E) of yeast cells (ste11Δ) transformed with STE11 alleles showing their ability to induce pheromone-dependent cell cycle arrest and transcriptional activation of mating-specific reporter gene.