FIGURE 5:

Structural mapping of functional data. (A) Mutagenesis data (Table 1) mapped on the modeled PH^Ste5 domain. Mutated residues are shown as CPK models color coded by activity change upon mutation relative to wild-type Ste5: red, <<0.1% mating activity and no Ste11 binding; orange, <1% mating activity and severely decreased Ste11 interaction; yellow, ∼10% mating activity and decreased Ste11 interactions. (B) Mutagenesis data (Table 2) mapped on the solution NMR structure of the RBL^Ste11 domain. Mutated residues are shown as CPK models color coded by activity change upon mutation relative to wild-type Ste11: red, <<0.1% mating activity; orange, <1% mating activity. Dashed lines indicate double mutants. (C) NMR interaction data mapped on the solution NMR structure of the RBL^Ste11 domain. CPK models indicate residues affected (in magenta, labeled) and not affected (in green, not labeled) upon binding to PH^Ste5 domain. Two orthogonal views are presented in each case corresponding to those in Figure 2.